Objective To observe effects of oxidative stress on streptozotocin（STZ） induced diabetic nephropathy（DN） rats and to probe the mechanism of Shenbaining（SBN）. Methods Left kidney of the STZ-induced DN rat was studied. Rats are separated into 4 groups：the sham operated group， the model group， the positive control group and the TCM group. The rats in TCM group are given 2. 97g·kg-１·d-１ SBN（dissolved in saline solution， prepared into suspension）. The positive control group is given 0. 9mg·kg-１·d-１ benazepril（dissolved in saline solution， prepared into suspension）. The model contrast group and the sham operated group are given the same volume of saline solution. After 12 weeks， 24h-Upro， MDA and T-SOD were measured in experimental rats. Results SBN can evidantly reduce the level of 24h-Upro （Ｐ<0.05）. Conclusions SBN can focus on tonifying kidney by against oxidative stress.