Capparis spinosaL. Inhibit the Invasion and Metastasis of Renal CellCarcinoma through Down-regulation of p-mTOR
Author:
Affiliation:
1. Zhejiang Chinese Medical University， Hangzhou 310053， China;2. The 117th Hospital of PLA， Hangzhou 310004， China;3. Hangzhou Emergency Medical Center,Hangzhou 310021,China
Objective To investigate the effect of Capparis spionosaL. on the proliferation， invasion and metastasis on the renal cell carcinoma 786-0 cells and reveal the mechanisms. Methods The cells were treated with different concentrations of Capparis spionosaL. The proliferation， invasion and metastasis were detected by MTT， Transwell assay and the level of mTOR and p-mTOR protein was detected by Western blot. Results Capparis spionosaL. inhibited the proliferation， invasion and metastasis of 786-0 cells depend on the time and drug concentration， 10， 20， 40ug/ml of the drug can significantly down-regulated the level of p-mTOR protein（P<0.05， P<0. 01， P<0.01）， but the drug has no effect on the expression of the mTOR protein（P>0.05）. Conclusion Capparis spionosaL. inhibit the proliferation， invasion and metastasis of 786-0 cells may through down-regulated the level of p-mTOR protein.