To conduct a preliminary study of Delphinium yunnanense Franch， and to build a theoretical basis for its medicinal identification， further research and development. Use macroscopic identification， microscopic identification， physical and chemical identification and TLC identification. Feature of Medicinal properties is surface brown， with curved vertical lines and root traces. The main microscopic characteristics：root cross cortex scattered stone cells and fibers and the layer is a ring . Xylem vessel are three bundles and arranged radially；many starch grains in power . The stone cell like square or rectangular， its cell wall is thickness， pits obvious . The main chemical composition is alkaloids and saponins. Four different herbs， which from different sources， in UV light （254nm）show same color fluorescent spots at the corresponding position， and the test had good reproducibility. The results of experiment can provide a theoretical basis for further development， utilization， and the formulation of quality standards.