Objective The purpose of this study aimed to investigate the anti-fatigue effect of Z. mays and P. oleracea and compatibility. Methods Mice were randomly divided into blank control group， Z. mays extract group， P. oleracea extract group， Z. ays， P. oleracea extract（1：2） compatibility group to crude drug was 500mg/kg.d-1， control group was given the same dose of saline administered continuously for 30 days， swimming time was measured in serum urea nitrogen and liver glycogen levels. Results with the blank group， each group can significantly prolong the swimming depletion time（P<0.05 or P<0.01）， increase glycogen content（P<0.05 or P<0.01）， and the compatibility group fatigue superior to single extract group（P<0.05）；but each group had no significant effect on serum urea nitrogen levels in mice after movement（P>0.05）. Conclusion Zea mays and Portulaca oleracea extracts have significant anti-fatigue effects in mice. Both share can obtain a better result， better than used alone.