Objective Response surface methodology was used to optimize the extraction conditions of polysaccharide from Morinda citrifolia. Methods Based on one factor test， the individual and interactive effects of ethanol concentration， material/liquid ratio， extracting temperature and extracting time on the yield of polysaccharide were studied. Results The experiment indicated that the optimal conditions include material/liquid ratio 1∶40， extraction temperature 100℃and extraction time 4h. Under these conditions， the yield of polysaccharide was 9. 677mg·g-1. Conclusion This exhibits the good feasibility of Response surface methodology for optimizing extracting process of polysaccharide from Morinda citrifolia.