Objective Establishing a HPLC method for the determination of scutellarein tetraacetate. Methods Column：Luna C18（4.6mm×150mm， 5μ）； Acetonitrile-0.02% phosphoric acid（50∶50） was used as the mobile phase at a flow rate of 1 mL/min， the detection wavelength was 254 nm， column temperature was 35℃. Results Methodology validation showed that the precision，stability，repeatability，linear range and average recovery rate all conforms to the requirement. Conclusion The method was so convenient， fast and accurate that it can be used in the assay and the quality control of scutellarein tetraacetate.