Objective To establish the oxidative damage model of SVAREC. Methods SVAREC were cultured in vitro and the growth curves were draw. Different concentration of H2O2 were stimulated the cells， and the cell viability were measured by the method of MTS at different time. Under the condition of this model， cellular morphology were observed by inverted microscope， the activity of LDH were measured by microplate reader， the apoptosis rate of cells also were detected by flow cytometry， and Caspase-3 expression were tested by Western blotting. Results Compared with control， cells were injured at the concentration of H2O2 is 100 μmol/L for 2 h， which， the apoptosis rate and protein expression of Caspase-3 were significantly higher. Conclusion The best replication model of this experimental condition is exposure the SVAREC at the 100 μmol/L concentration of H2O2 for 2 h.