Objective To investigate the effect of Shunqi Tongfu mixture on plasma MTL， Ach， SS in rats with gastrointestinal motility disorder. Methods Select SD rats were randomly divided into normal control group， negative control group， Shunqi Tongfu mixture group and mosapride citrate group. Except the normal control group， the other three groups were prepared into models of gastrointestinal motility disorder， then given drug administered， abdominal aortic blood， Elisa determination of plasma MTL， Ach， SS content. Results ①After treatment， the weight of Shunqi Tongfu mixture group was significant difference from the negative control group（P<0.05）. ②Model group MTL， Ach， SS were significantly difference from the normal control group（P<0.05）. After treatment， Shunqi Tongfu mixture group MTL， Ach in plasma increased significantly and SS decreased significantly， all compared with negative control group（P<0.05）. Conclusion The mechanism of Shunqi Tongfu mixture promote gastrointestinal motility may be： Elevate levels of plasma MTL and Ach， then promote the effect of gastrointestinal smooth muscle contraction；inhibit the secretion of SS， promote gastrointestinal and pancreatic exocrine secretion， activate the protective effect on the gastrointestinal mucosa， maintain the normal physiological function of gastrointestinal tract.