Objective To observe effect of YinZhi anorectal smoked lotion acting on the hemorrhoid tissue cytokines factor VEGF、CD68 and MMP9. Methods Selected 40 cases of acute episode of hemorrhoid patients were randomly divided into 20 patients in each treatment group and the control group. The treatment group were cured with YinZhi anorectal smoked lotion and the control group were cured with Ma Ying-Long Jin Xuan hemorrhoid washing powder， All two group were fumigated partialy on anal after 7 consecutive days. The hemorrhoid tissue gotten from patients compare with that were cured through surgery directly without medication by immunohistochemical staining examination. Observe the effect of medication on hemorrhoid tissue cytokines factor VEGF、 CD68 and MMP9. Results After medical treatment， YinZhi anorectal smoked lotion treatment group and Ma Ying-Long Jin Xuan hemorrhoid washing powder control group is better than blank group about inhibiting VEGF， CD68， MMP9 expression（P<0.01）； YinZhi anorectal smoked lotion treatment group is better than Ma Ying-Long Jin Xuan hemorrhoid washing powder control group（P<0.01/0.05）. Conclusion YinZhi anorectal smoked lotion inhibits expression of hemorrhoidal tissue cytokines factor VEGF、CD68 and MMP9. The therapeutical effect of YinZhi anorectal smoked lotion acting on hemorrhoidal tissue is possibly related with expression of inhibiting VEGF， CD68， MMP9.