Objective To investigate the mechanisms of total Flavonoid in Hickory Leaves on anti-myocardial ischemia in mice and the protective study of hypoxia injury of myocardial cells（H9c2）. Methods 1. Establish the model in mice.subcutaneous injection of hydrochloric acid isopropyl adrenaline cause acute myocardial ischemia； hearts slicing； HE staining； and determine contents of the Creatine Kinase（CK）、 lactate dehydrogenase（LDH）、 Superoxide Dismutase（SOD）、 Malondialdehyde（MDA） in mice serums. 2. Establish the model of hypoxia injury of myocardial cells injury by H2O2； observe cells morphology； determine cells survival rate and contents of the Creatine Kinase（CK）、 Superoxide Dismutase（SOD）、 Malondialdehyde（MDA） in the cells culture medium. Results Compared with the model group， total flavonoid could decrease inflammatory cytokines in the myocardial tissue， could effectively decrease serums LDH、 MDA and CK enzymic activity， increase the level of SOD. The total flavonoid could significantly increased the viability of myocardial cells， inhibited the release of LDH， decreased the content of MDA， increased SOD activity. Conclusion The total flavonoid in hickory Leaves has protective effects on myocardial ischemia in mice and hypoxia injury of myocardial cells.