Objective To investigate the effect of Fumai decoction on PKC and P-Cx43 S368 in arrhythmia rat. Methods Divided the rats into sham operation group， model group， metoprolol group（104mg·kg-1）， group of Zhigancao decoction， low dose， middle dose and high dose groups of Fumai decoction. Rats of myocardial ischemia model were induced by left anterior descending ligation for one hour.After the treatment of Fumai decoction， the PKC， Total-Cx43 and P-Cx43 S368 expression was characterized by Westernblot. Results Compared with sham operation group， all the PKC， Total-Cx43 and P-Cx43 S368 expression of model group were significant reduced（P＜0.01）. Compared with model group， all the PKC， Total-Cx43 and P-Cx43 S368 expression of middle dose and high dose groups of Fumai decoction and metoprolol group were significant increased（P＜0.01）. PKC， Total-Cx43 and P-Cx43 S368 of group of Zhigancao decoction and low dose group of Fumai decoction also expressed higher than model group（P＜0.05）. Conclusion Fumai decoction can increase the PKC， Total-Cx43 and P-Cx43 S368 expression in myocardial ischemia rats， increase the gap junction channel permeability and conductance， and decrease the level of myocardial injury. This may be one of the mechanism of Fumai decoction to treat arrhythmia in myocardial ischemia rats.