Objective To observe how the transcutaneous electrical acupoint stimulation effects on of serum lactate and creatine kinase of the college athletes of intensive physical training. Methods 45 male college athletes were randomly divided into the control group， the treatment group 1 and the treatment group 2. 15 people in each group. Each group was training intensively for 2 weeks， evaluating physical subjective feelings and testing athletes values of serum lactate and creatine kinase of each group before and after training respectively. Results Compared with the serum lactate and creatine kinase of the athletes of control group before and after the experiment， there was very significant difference（P<0.01）. Compared with the serum lactate and creatine kinase of treatment group 1 and 2 before and after the experiment， there was significant difference（P<0.05）. After the experiment， compared with the serum lactate and creatine kinase of control group， there is a significant difference（P<0.05） between the treatment group 1 and 2. The values of the physical subjective feelings of each group compared with the values before the experiment also have a very significant difference（P<0.01）； after the experiment， the subjective physical feeling values of the treatment group 1 and 2 have significant differences（P<0.05） compared with the model group；compared with the treatment group 1 and 2 there is no significant difference（P>0.05）. Conclusion Excessive exercise training can cause our body fatigue and increase the content of serum lactate and creatine kinase. Transcutaneous electrical acupoint stimulation can improve the state of fatigue and enhance athletic ability， as well as reduce the the content of the serum lactate and creatine kinase. The transcutaneous electrical acupoint stimulation has a role in preventing and treating exercise-induced fatigue.