Objective To develop a HPLC fingerprints of Glycyrrhiza uralensis Fisch. Methods The HPLC method was adopted on ZORBAX Eclipse XDB-C18（4. 6 mm × 250 mm， 5 μm） analytical column by gradient elution with acetonitrile and phosphoric acid water solution（0.05%） as mobile phase. The analysis time was 70 min and the detection wavelength was 215 nm（0-16 min）， 360 nm（16-22 min） and 250 nm（22-70 min）. Results The HPLC standard fingerprint chromatogram of Glycyrrhiza uralensis Fisch. which consists of 7 common characteristic peaks. The results of method validation accorded with the technical standard of fingerprints. Conclusion The method is stable， accurate， reliable， and can provide a scientific basis for the quality control of Glycyrrhiza uralensis Fisch.