Objective To explore the effect of exogenous substances on seed germination， and provide technical guidance for seed breeding of Paris polyphylla. Smith var. yunnanensis. Methods The seeds of P. polyphylla. Smith var. yunnanensis treated with different reagents at various concentrations of GA3、 NAA、 IAA、 6-BA、 KNO3 and H3BO3， then the seed germination was condition of studied. Results The germination rate of P. polyphylla. Smith var. yunnanensis seed was improved significantly by high concentrations（200、 500mg/L） of GA3， 20mg/L IAA and low concentrations（10、20mg/L） of KNO3. The germination rate of seed was also improved significantly by the low concentration（10mg/L） 6-BA， however， it was inhibited by the high concentration（50、 100mg/L） 6-BA； The germination rate of seed was also inhibited by the NAA and high concentration（500mg/L） H3BO3. Conclusion The effect of exogenous substances on seed germination has close relationship with its concentration.