Objective To explore the therapeutic efficacy of mild reinforcing-attenuating needle pricking therapy in treatment of azoospermia and sex hormone. Methods Randomized into treatment group and control group， to explore the different of the semen analysis and the sex hormone between two groups. Results After two month’s treatment， the semen analysis and sex hormone in treatment group took a turn for the better， and closer to the record of control group. Conclusion Mild reiforcing-attenuating needle pricking therapy in treatment of azoospermia is by regulating the sex hormone’s bidirectional secretion.