Objective To observe the protective effect of serum containing LGZGD on myocardial H9c2 cells injury induced by TGF-β1 in vitro. Methods The myocardial H9c2 cells were incubated with serum containing LGZGD respectively（100 μL、10％）. After 12 hours， myocardial H9c2 cells in each group were treated with TGF-β1（20 ng·mL-1） except normal control group. The method of ELISA was used to detect the contents of TNF-α、IL-6 and IL-1β. Results Compared with the normal control group， the contents of TNF-α、 IL-6 and IL-1β increased markedly（P<0.01）. Compared with TGF-β1 group， the content of TNF-α in LGZGD 4.2， 8.4 g·kg-1 pretreatment groups declined from （323.839±35.614） to （191.399±43.203） and （175.030±19.589）ng·L-1（P<0.05、 P<0.01）， and the IL-6 content declined from （67.171±13.506） to （40.490±7.899） and （30.548±2.226）ng·L-1（P<0.05、P<0.01）； the content of IL-1β decreased to （14.793±4.244）、 （14.600±3.517） and （9.253±2.720）ng·L-1（P<0.05、P<0.01） in LGZGD 2.1， 4.2， 8.4 g·kg-1 groups. Conclusion Serum containing LGZGD has significant protective effects on myocardial H9c2 cells injured by TGF-β1.