Objective To explore the influences of Gandouling on liver injury of copper-laden rats. Methods The tested rats were randomly divided into the control group， model group and Gandouling group. After modeling， TUNEL method was applied to test the apoptosis cells index and NMR-based metabonomics was applied to analyze the metabolic changes of rat kidney tissue. Results Compared with the control group， the apoptosis cells index in kidney of the model group was significantly increased（P<0.01）； the content of phenylalanine， alanine， tyrosine， glutamate， lysine， valine， leucine， inosine and aspartate was decreased. Compared with the model group， the apoptosis cells index in kidney of the Gandouling group was significantly decreased（P<0.01）； the content of the above metabonolates was increased. Conclusion There is certain intervention influence of Gandouling on copper-laden rats. The related mechanism may involve regulating aromatic amino acid（phenylalanine， alanine） and branched chain amino acid（valine， leucine） metabolism， inhibiting apoptosis of renal tubule epithelia and regulating ammonia metabolism.