Objective To observe the effect of Ginsenoside-Rg3 on the expression of interleukin-1β（IL-1β） and tumornecrosis factor-α（TNF-α） in the ear wounds. Methods Collagen I and III expression detected by immunohistochemistry and scar elevation index（SEI） was used to evaluate the extent of scarring. The expression of inflammatory factors interleukin-1β（IL-1β） and tumor necrosis factor-α（TNF-α） was detected by immunohistochemistry. Results Compared with control scars， Ginsenoside-Rg3-treated wounds exhibited decreased inflammation significantly as evidenced by the lower levels of IL-1β and TNF-α. The SEI and the synthesis of collagens I and III were significantly decreased in the Ginsenoside-Rg3-treated scars（P<0.05）. Conclusion Immediate topical injection of Ginsenoside-Rg3 during the wound healing process can reduce the severity of hypertrophic scarring in a rabbit model， which may result from controlling inflammation， inducing fibroblast apoptosis and promoting collagen degradation by decreased the inflammatory factors of IL-1β and TNF-α.