Objective To observe the effect of chamomile extract to normal mice’ blood glucose and glucose tolerance. Methods Mice were divided into two groups：normal and model group. Normal mice were divided into 8 parts， they were blank， Ys high（200mg）， Ys middle（100mg）， Ys low group（50mg）， Yc high（200mg）， Yc middle（100mg）， Yc low group（50mg） and metformin（positive control group 110.5mg）， there were 5 mice in each group. Each group was respectively administrated the corresponding component or distilled water， for 7 days， blood glucose and oral glucose tolerance text（OGTT） were measured to observe the effect of chamomile extract on blood glucose of normal mice， and observe the ameliorative effects of chamomile extract on glucose tolerance of normal mice. Results Chamomile extract can reduce the blood glucose level of fasting mice dramatically than metformin（positive control group）. Yc medium dose is best， that the effect of each group was much better than metformin（positive control group）. The best effect of aqueous extract is Ys large dose and showed a certain dose-effect relationship. Chamomile extract can ameliorate the glucose tolerance level of normal mice， better than metformin（positive control group） also. Conclusion Chamomile extract has hypoglycemic effect on the normal mice， and can be used as treatment on diabetes in clinical.