Objective Optimization the extraction technology of total phenolic compounds from the spine grape peel， and determine its antioxidant activity. Methods The extraction technology of total phenolic compounds from the spine grape peel was studied by single-factor experiments and orthogonal test design. Furthermore， the antioxidant activities of spine grape peel were compared with Vc（or EDTA）， using various antioxidant assays included the scavenging on ABTS+， DPPH and OH free radicals， ferrous ion-chelating capacity and influence on lipid peroxidation in rats. Results The optimum extraction conditions determined by orthogonal experiment as follows：ethanol concentration 40%， solid-liquid ratio was 1∶14， ultrasonic power 225 W and extraction time 40 min. The extraction quantity of total phenolic compounds under this condition was 16. 823 mg/g. It has strong scavenging activity on ABTS+ and DPPH， while weak scavenging activity on OH free radicals， ferrous ion-chelating capacity and inhibiting the brain lipid peroxidation in rats. Conclusion This study suggested that spine grape peel has antioxidant activity under the optimal extraction technology， but it’s weaker than Vc.