Objective To prepare and identify HP-β-cyclo dextrin（β-CD ）inclusion compound of Cang Ai volatile oil. Methods With the inclusion rate of Cang Ai volatile oil-β-CD complex and the yield of volatile oil HP-β-CD complex as evaluating indicators， saturated water solution method was investigated. Orthogonal design was used to select the optimum formulation. The volatile oil HP-β-CD complex was identified by DSC， UV and TLC. Results The optimum inclusion conditions were as follows：volatile oil：HP-β-CD in proportion as 1∶15（mL∶g）， inclusion temperature of 40℃， mixing time of 3h， analysis results of DSC and TLC showed that stable inclusion compound has been formed from volatile oil and HP-β-CD. Conclusion The optimum condition for inclusion was feasible， solubility and stability which were improved after incusing by HP-β-CD. The comprehensive evaluating indicators may provide scientific basis for both screening the technological process and quality control.