Objective To determine the content of the injection Breviscapine by UV spectrophotometry and high performance liquid chromatography. Methods UV spectrophotometry using methanol as solvent， choose 335nm as wavelength. High performance liquid chromatography using Luan C18（150×4.6nm） as analytical column and the mobile phase was 0.05% phosphate water-acetonitrile gradient Elution. The column temperature was 35 ℃， the flow rate was 1mL/min， the detection wavelength was set at 335 nm. Results UV spectrophotometry， Breviscapine in 5. 0～15. 0μg/mL within the range of absorbance was linear（r=0.99985）， the average recovery was 99.20%（n=9）， RSD=0.82%（n=9）， precision and repeatability RSD good， respectively for 0. 15% and 0. 93%； HPLC Breviscapine in 10.0～80.0μg/mL range and peak area showed a good linear relationship， r=0.9998， the average recovery was 100.69%. Conclusion Although UV spectrophotometry is simple， fast， but specificity is poor， susceptible to external environmental impacts. Therefore， considering the selective determination injection comparative advantage Breviscapine content by HPLC.