Objective To investigate the effects of the panax notoginseng sapoins（PNS） on brain vascular endothelial cells apoptosis and expression of TGF-β、 VEGF， in order to reveal the molecular mechanism of PNS on brain protection. Methods The model of hypoxia induced damage in cultured brain vascular endothelicocyte cells was set up in vitro and divided into normal group， model group and PNS group. Flow cytometry was adopted to detect cell apoptosis， ELISA was used to determine the protein level of TGF-β1， VEGF-B， the expression of TGF-βwas measured by western blot. Results Compared with the normal group， the rate of apoptosis in model group increased significantly（P<0.01）； Compared with the modell group， the rate of apoptosis in PNS group decreased significantly（P<0.01）. Compared with the normal group， the level of TGF-β1 increased significantly in model group（P<0.05）. In model group the level of VEGF-B increase significantly（P<0.01） compared with the normal group； compared with the model group， the level of VEGF-B in PNS group increased（P>0.05）； the expression of TGF-βis less in normal group， expression of TGF-β in model group increased（P<0.05）， compared with model group， the expression of TGF-β is decreased. Conclusion PNS can decrease the rate of brain vascular endothelial cells apoptosis， and inpaired the damage of cells， beneficial to angiogenesis， so as to protect the neurons in hypoxia injury.