Objective To establish an HPLC method for determining the content of Kaempferol-3-O-rutinoside in Fengliao Changweikang Tablets. Methods Using US High Performance Liquid Chromatograph Instrument and acetonitrile-0. 4%formic acid water solution（16∶84） as the mobile phase， the flow rate was 1. 0mL/min and test wavelength was 346nm and the column temperature was 35℃. Results Kaempferol-3-O-rutinoside had a good linearity within the range of 6.92～69.2μg·mL-1（r=0. 9997）， the average recovery was（n=6） 98.69%. Conclusion This methods can be use as a measure to determine the exact content of Kaempferol-3-O-rutinoside in Fengliao Changweikang tablets and as a control over product quality as well.