Objective To discuss the change of oxidative stress in Asthenia-cold syndrome of primary dysmenorrhea in serum， and complete the interventional mechanis of Jin Gui Wenjing decoction to curing the disease. Methods Collected 60 cases with Asthenia-cold Syndrome of Primary Dysmenorrhea， curing with Jingui Wenjing decoction. Collected 60 cases of normal women as control group. Compared the changes of serum reproductive hormones（FSH、LH、E2、P、T） and oxidative stress index（SOD、MDA、GSH-PX、T-AOC） between before and after treatment. Results Dysmenorrhea patients before treatment， the serum E2， SOD， GSH-Px and T-AOC were significantly lower， and MDA increased， compared with normal group， the difference statistically significant（P<0.05 or P<0.01）. After treatment， serum E2， SOD， GSH-Px and T-AOC also increased to different extent， MDA decreased， compared with before treatment， statistically significant difference（P<0.05 or P<0.01）； And E2， SOD activity and MDA after treatment compared with normal group， there was no statistically significant difference（P>0.05）. Conclusion Asthenia-cold Syndrome of Primary Dysmenorrhea have oxidative stress injury； Jingui Wenjing decoction can effectively treat the asthenia-cold syndrome of primary dysmenorr-heal by activating the body’s antioxidant ability to alleviate the body state of oxidative damage.