Objective Provide experimental basis through observing inhibit activity of DPP-4 and α-glucosidase with flaxseed water extraction liquid. Methods 1. Established in vitro screening system of DPP-4 inhibitor by using DPP-4 enzyme，buffer solution and substrate. Then carried on suppression experiment of flaxseed water extraction liquid and determined absorbance through chromogenic peptide substrate method. Finally，calculated the DPP-4 suppression rate and the IC50 value. 2. Took sucrose as substrate to establishα-glucosidase enzyme activity inhibition model. Then determined inhibitory effect of flaxseed water extraction liquid on α-glucosidase enzyme. Finally，calculated the inhibition rate and IC50 value. Results 1. Flaxseed can mildly inhibit DPP-4，its IC50 value was 738.20mg/L. 2. Flaxseed can inhibit α-glucosidase enzyme，its IC50 value was 365.9mg/mL. Conclusion Flaxseed water extraction liquid caninhibit the activity of DPP-4 and alpha glycosidase enzyme to a certain degree.