Objective Establishing a HPLC method for the determination of monoester aconitum alkaloids in paste of Fuqi Guben. Methods Column Waters XTerra RP18 5mm（4.6mm×250mm）； column temperature was 30℃； Acetonitrile -tetrahydrofuran（25∶15） as the mobile phase A， 0.1mol/L ammonium acetate solution（1000mL containing glacial acetic acid per 0.5mL） as the mobile phase B， gradient elution， flow rate was 1 mL/min， the detection wavelength was 254 nm. Results Benzoylmesaconine is in the range of 0.045~1.800μg showed a linear relationship， benzoylaconitine is in the range of 0.0425~1.7000μg showed a linear relationship， and benzoylhypacoitine is in the range of 0.0442~1.7680μg showed a linear relationship. Precision， stability， reproducibility are in line with the requirements， the average recovery of this measurement method were 99.89%， 99.22%， 101.12%， RSD was 1.72%， 1.75%， 2.32%（n=6）. Conclusion The method was so convenient， fast， accurate and no interference that it can be used for quality control of the preparation.