Objective To establish the methods for determination the content of Pb， Cd， Cu， As and Hg in Piceatannol-3’-O-β-D-glucopyranoside from different batches so as to determine the residue of five elements. Methods Samples were digested with microwave digestion system； the content of Pb and Cd was measured by GFAAS（graphite furnace atomic absorption spectrometry）， the content of As and Hg was measured by HG-AFS（atomic fluorescence spectrometry）， the content of Cu was measured by FAAS（flame atomic absorption spectrometry）， and the measurement methods were methodologically evaluated. Results The recovceries（n=9） of Pb， Cd， Cu， As， and Hg were 95.18%， 96.29%， 101.32%， 97.88%， 86.76%， respectively. Conclusion These methods are simple and rapid for operating and can be used for the determination of total Pb， Cd， Cu， As and Hg in pharmaceutical raw materials.