Objective To investigate the anti-inflammatory and analgesic of the gout one prescription， and to improve the experimental evidence for clinical treatment of acute gout arthritis. Methods We need to prepare to ear swelling model of rats by xylene and the capillary permeability induced by acetic acid of rats， to observe the anti-inflammatory effect of the gout one prescription；the experimental pain model of rats induced by acetic acid and hot pate method was used to observe the analgesic effect of the gout one prescription. Results The effect of Ⅱgroup and Ⅲ group of the gout one prescription on the ear swelling of rats was significantly inhibited（P<0.05， P<0.01）；the increasing of the peritoneal capillary permeability in mice induced by acetic acid was significantly inhibited by Ⅱgroup and Ⅲ group of the gout one prescription（P<0.01）；the analgesic effect of I group、Ⅱgroup and Ⅲ group of the gout one prescription on the mouse torsion model induced by acetic acid was significant（P<0.01）， and the analgesic effect of I group、Ⅱgroup and Ⅲ group of the gout one prescription has dosage dependence；30minutes， 60minutes， 90minutes， 120 minutes after given by Ⅱgroup and Ⅲ group of the gout one prescription， its incubation period of pain response in mice was prolonged（P<0.05， P<0.01）， and the pain threshold was increased at a rate above 25%. Conclusion The gout one prescription has a demonstrable effect on anti-inflammatory and analgesic， and it can be used for the treatment of acute gout arthritis.