Objective To establish the TLC identification method and HPLC fingerprint of Dysosma versipellis to provide better specificity test method for the quality control. Methods The sample solution was prepared by ultrasonic extraction with ethyl acetate， and the chloroform-methanol-water（9∶1∶0.1）was used as an expansion system， and the development of 13cm， 10% sulfuric acid ethanol solution was used as the chromogenic reagent；Thermo AcclaimTM 120 C18（4.6×250 mm， 5μm）was used with acetonitrile-0.05% phosphoric acid water solution as mobile phase in a gradient mode at the flow rate of 1. 0mL/min. The detection wavelength was 203 nm and the temperature of column was set at 36℃. Similarity evaluation system for chromatographic fingerprint of TCM was applied to analysis different batches of Dysosma versipellis samples. Results The TLC identification method and HPLC fingerprint of Dysosma versipellis was established， eleven mutual peaks which separation in good condition were selected as the fingerprint peaks in 10 batches sample. Conclusion This method is simple and reliable， can be used for the identification and quality control of Dysosma versipellis.