Objective To establish a method for determination of five saponins in Notoginseng and Sanqi Panax Notoginseng Injection by ultra-performance liquid chromatography（UPLC） method. Methods The determination was carried out on Waters Guard column（50mm×2.1mm， 1.7μm）. The mobile phase consisted of [water-acetonitrile] -acetonitrile with gradient elution at a flow rate of 0.5 mL/min. The detection wavelength was set 203 nm and the column temperature was at 25℃ with injection volume of 2μL. Results Notoginsenosides had good linearity in the ranges of 0.002～0.817μg（r＝0.9999）. The average recovery was 101.5% with RSD of 1.31%. Conclusion The method has simple operation， accurate results， good reproducibility， and specificity which can be used in the quantity control of Notoginseng total saponins in Notoginseng and Sanqi Panax Notoginseng Injection effectively.