Objective To study about Strong Bone Pain Side All action on osteoporosis female on the expression of receptor activator of NF-κB（RANK）， receptoractivator of NF-κB ligand（RANKL） and osteoprotegrin（OPG）. Methods Rat was randomly divided into five groups：control group， OP model group and Strong Bone Pain Side All high dose treatment group， Strong Bone Pain Side All lower dose treatment groups， estradiol group. Determination of OPG and RANGKL in rat serum by ELISA. Results Serum OPG of model group rats decreased and RANGKL levels increased， rats serum OPG levels increased and RANGKL levels decreased significantly by strong Bone Pain Side All intervention. Conclusion Strong Bone Pain Side All can reduce rats serum RANKL/OPG ratio， activation of RANKL/RANK/OPG signal system， achieve the prevention and treatment of osteoporosis.