Objective To analyze the appearance differences of Dysosma versipellis， and explore the relevance between the appearance differences and qualities. Methods The plant and medicinal traits of Dysosmaversipellis from 12 habitats were measured as index. The effective components content of podophyllotoxin， quercetin and kaempfero were determined by HPLC. The data were analyzed by SPSS 19. 0 software. Results The leaf division， root weight， root length， root diameter and lateral root number of 12 habitats had significant differences. Podophyllotoxin content was positively correlated with root diameter， leaf division and was negatively correlated with habitat， root number. Quercetin content was positively correlated with dry weight per plant and root section number. Kaempfero content was positively correlated with root section number and lateral root number. Conclusion There are significant individual differences in Dysosmaversipellis and significant differences between mid-eastern and southwestern regions. The content of active ingredients has a certain correlation with the plant and medicinal traits.