Objective To establish a method for determination of matrine and Oxymatrine in Tongfengqing Lotion. Methods The content of matrine and oxymatrine was determined by HPLC on Dikma Platisil NH2 column（250mm×4.6mm， 5μm） with a mobile phase of acetonitrile-absolute alcohol-2% phosphoric acid solution（75∶12.5∶12.5， v/v）， the flow rate was 1.0 mL·min-1， and the detection wavelength was set at 220 nm， the injection volume was 10 μL， the column temperature was being 30 ℃. Results The calibration curves of matrine， oxymatrine were in good linearity over the ranges of 0. 784~9. 408 μg（r=1.0000）， 0.484~5.808 μg（r=0.9999）， and the average recovery of matrine and oxymatrine was 100.99％， 99.08% with RSD of 1.63％， 1.89%（n=9）. Conclusion The method is simple， quick， accurate， specific and reproducible for the quality control of TongFengqing lotion.