Objective To evaluate the effect of Jianpi Qingchang Decoction containing serum （JQD-CS） on proliferation of mice intestinal interstitial cells of Cajal（ICC） in vitro. Methods Cultivation and identification of mice intestinal ICC in vitro；preparation of JQD-CS. MTT method detected the proliferation of ICC intervened by different concentrations of JQD-CS. Results Moderate concentration（less than 20%） of the JQD-CS， the OD value of ICC increase with the increasing concentration of serum. Conclusion Moderate concentration of JQD-CS can promote the proliferation and differentiation of ICC.