Objective To study the expression of tumor associated macrophages microRNA in lung cancer microenvironment spleen deficiency syndrome and the influence of modified HuangqiJianzhong soup. Methods Using bitter and cold， diarrhea， weary， dieting methods of modeling in 50 mices， after the establishment of mouse spleen deficiency syndrome and Lewis lung cancer model， according to the experimental design of the processing of each group of mice， after modeling Huangqi Jianzhong decoction gavage， the mice were killed off the cervical spine， and tumor tissue was collected. Use MicroRNA gene chip for the detection of variations of normal mice macrophages and three groups of tumor tissue macrophage expression of MicroRNA gene. Results MicroRNA microarray detection of tumor associated macrophages of mice found， Spectrum expression of Spleen Qi deficiency group tumor associated macrophage microRNA（compared with the normal group）aberrant， unlike tumor group， has a unique MicroRNA changes， and modified Huangqi Jianzhong decoction can significantly bidirectional regulate “Spleen Qi Deficiency” abnormal changes in expression profiles of tumor-associated macrophages microRNA tissue to make it back to normal. Conclusion The modified Huangqi Jianzhong decoction can make “Spleen Qi Deficiency” microRNA back to normal.