Objective The objective of this study was to investigate the relationship between chronic HBV infection and gallbladder abnormal， by doing this to probe the theory that liver and gallbladder being closely related. Methods Take B mode ultrasound images examination for every chronic HBV infect patient， calculate the differences of abnormal gallbladder rates between sex age and duration with chronic HBV infection. Results ①No statistical significance was found in sex P>0.05；②Abnormal gallbladder rates increased as the age goes P<0.05；③Abnormal gallbladder rates increased as the duration with chronic HBV infection goes P<0.05. Conclusions Patients with chronic HBV infection come to a higher abnormal gallbladder rate than healthy people；Longer duration with chronic HBV infection， higher abnormal gallbladder rates. The results can present the intimate relationships between liver and gallbladder in Traditional Chinese Medicine that persist illness of liver can influence the functions of gallbladder. Coincide with liver and gallbladder being closely related theory. So we put the hypothesis that add promoting bile secretion beside dispersing the stagnated liver-qi therapy may receive a better effect in curing chronic HBV infection patients.