Objective To investigate the chemical constituents from the whole plant of Isodon lophanthoides var. graciliflora which collected in Guangdong， with a view to discovering the lead compounds with pharmacological activity. Methods The chemical constituents were isolated and purified by the combination of macroporous adsorption resin， silica gel， Sephadex LH-20 and ODS column chromatography and preparative high performance liquid chromatography. Their structures were elucidated on the basis of physicochemical properties and nuclear magnetic resonance spectroscopy data. Results Seven known compounds were isolated from the acetone extract of Isodon lophanthoides var. graciliflora. They are 2-hydroxydiplopterol（1）；ursolic acid（2）； uvaol（3）； stigmasterol（4）； β-sitosterol（5）；dibutyl terephthalate（6）； dipropyl octadecanedioate（7）. Conclusion Seven compounds were isolated and identified in the process of studying the chemical constituents of the whole plant. Among them， compounds 1-3 are triterpenoids， compounds 4 and 5 are sterol type， compounds 6， 7 are structurally symmetrical ester type， and 1-3， 6， 7 are isolated from the plant for the first time.