Objective To compare the contents of different parts of Salvia miltiorrhiza in different cultivation methods and to select the reasonable cultivation mode. Methods According to the method described in the Chinese Pharmacopoeia 2015 Edition， using high performance liquid chromatography（HPLC） to determinate the content of salvianolic acid B and tanshinone（Salvia miltiorrhiza of tanshinone IIA， cryptotanshinone， tanshinone I） which come from growning on different latitude in different breeding cultivation， rooted in two different planting Danshen root， stems， leaves， receptacle， fibrous roots. Results There were significant differences in different parts of Salvia miltiorrhiza content， plant Salvia miltiorrhiza and rooted salvia miltiorrhiza， tanshinones and salvianolic acid B in different parts of the distribution of content are root > fibrous roots > receptacle > stem > leaf； tanshinone content in root and stem rooted in Salvia miltiorrhiza was higher than that of plant Salvia miltiorrhiza. The content of salvianolic acid B in plant Salvia miltiorrhiza of five parts were higher than the content in rooted salvia miltiorrhiza. Conclusion Salvianolic acid B in plant Salvia miltiorrhiza was higher than that in rooted salvia miltiorrhiza， but tanshinone was lower in rooted. Salvia miltiorrhiza cultivation methods can be a reasonable choice depending on the desired composition， guide rational planting.