Objective To study the acute toxicity and analgesic and anti-inflammatory effects of the ethnol extraction of Lepidogrammitis drymoglossoides（Baker） Ching in mice. Methods The acute toxicity was observed by maximum tolerance dose（MTD）experiment in mice， observed analgesic effect in mice by writhing reaction induced by glacial acetic acid， and to study the anti-inflammatory effect using the model of auricle edema in mice induced by xylene. Results The minimum lethal dose of the ethnol extraction of Lepidogrammitis drymoglossoides（Baker） Ching in mice is 30.91g/kg·bw， and the maximum tolerance dose of that is 24.73g/kg·bw. The ethnol extraction of Lepidogrammitis drymoglossoides（Baker） Ching could inhibit writhing pain induced by glacial acetic acid， and also could inhibit auricle edema induced by xylene in mice. Conclusion The minimum lethal dose of the ethnol extraction of Lepidogrammitis drymoglossoides（Baker） Ching in mice is 30.91g/kg·bw， and the maximum tolerance dose of that is 24.73g/kg·bw. The ethnol extraction of Lepidogrammitis drymoglossoides（Baker） Ching has significant analgesic anti-inflammatory effect.