Objective To research the expression rates of TIGIT， CD226， CD96 in the peripheral blood of AA patients and the relationship between them and Chinese tradition medicine type. Methods 60 AA patients and 20 normal persons were enrolled in the research. The AA group was devided into PISHENYINXU and PISHENYANGXU. The percantage of TIGIT， CD226， CD96 in he peripheral blood were determined by flow cytometry（FCM）. Results In AA patinets group， the proportions of TIGIT decreased and the CD226， CD96 expression rate was higher than the normal group . The CD226， CD96 expression rate in the PishenYinxu group was higher than it in PishenYangxu group. The TIGIT expression in the patients that the duration was less than one year was less than that in the patients that the duration was longer than one year. The results were significantly different（P<0.05）. Conclusion It is suggested that the costimulatory molecules TIGIT， CD226 and CD96 play a role in the pathogenesis of AA. The expression of them has difference on basis of the TCM different syndrome type. The expression of them has relationship with the course of disease.