Objective Differences in the effects of Taohong Siwu Decoction HPLC characteristics of different extraction method and the comparison of different extraction methods on the chemical composition change. Methods The characteristic chromatogram was obtained on a Welch Materials C18（4.6 nm×250nm， 5μm） eluted with a gradient of acetonitrile and water（include 0.05% phosphoric acid）， at a flow rate of 1 mL·min-1， with wavelength switching detection， the column temperature was 40°C and sample volume was 25μL. Results The characteristic spectrum of traditional water extraction and 90% ethanol-water extraction of Taohong Siwu Decoction were established respectively， and the common peaks areas and numbers were quite different. Conclusion The characteristic chromatogram established were workable with remarkable repeatability， and can be used as the reference for quality control， process research and correlation of composition and efficacy of Taohong Siwu Decoction.