Objective To observe the effect of periodic acupoint catgut implantation on the quanity of estrogen E2 and progesterone P in rats model of Hyperplasia of mammary gland. Methods 32 Female non-pregnant SD Rats were randomly divided into normal control group， model control group， periodic acupoint catgut embedding group and common acupoint catgut embedding group. The treatment group was embedded 1 time in each sexual cycle， 1 times for a course， and a total of 3 sexual cycle. After the intervention， blood samples were drawn from the abdominal aorta， separate the serum， then test the quanity of E2 and P by using the electrochemical immunoluminometric. Results Compared with the model control group， serum E2 level of periodic acupoint catgut embedding group was significantly decreased， and the serum P level was significantly increased， and there were significant differences（P<0.01）； The serum E2 level of the common acupoint catgut embedding group was significantly reduced， and the serum P level increased significantly， and there were significant differences（P<0.05）. Compared with the common acupoint catgut embedding group， the decrease of serum E2 level and P level in the rats with periodic acupoint catgut embedding group was more obvious， and there was significant difference（P<0.05）. Conclusion the periodic Catgut embedding method and the common acupoint catgut embedding method can inhibit and improve the hyperplasia of mammary gland， but the effect of the periodic acupoint catgut embedding method is better than the common acupoint catgut embedding method， which may be related to the regulation of serum sex hormone level.