Objective The establishment of rabbit cultured KOA cell line in vitro， to observe the effect of Kangfuxin Liquid on the proliferation of rabbit chondrocytes KOA and the effect of Kangfuxin Liquid on the expression of p44/42MAPK protein in rabbit chondrocytes in KOA. Methods The rabbit knee osteoarthritis was obtained by enzyme digestion method， and the third generation rabbit knee osteoarthritis cells were studied. The Kangfuxin Liquid in different concentrations were added to the third generation of rabbit knee osteoarthritis cells， evaluation of effects on the proliferation of rabbit KOA chondrocytes by CCK8 method；the expression of p44/42MAPK Western blotting KOA detection of rabbit chondrocytes in the protein. Results 20mol/L， 15mol/L， 10mol/L Kangfuxin group on rabbit chondrocyte KOA inhibition level than the DMSO control group， the differences were statistically significant（P<0.05）； 5mol/L， 10mol/L， 20mol/L Kangfuxin Liquid to p44/42 MAPK KOA of rabbit chondrocytes in protein expression level than the control group， the differences were statistically significant（P<0.05）； 15 mol/L group p44/42 MAPK of Kangfuxin Liquid in cartilage cells of rabbit KOA protein expression levels than the control group， the difference was statistically significant（P<0.01）. Conclusion Kangfuxin Liquid may inhibit rabbit KOA cartilage cells promote the expression of p44/42MAPK protein in rabbit KOA in chondrocyte proliferation.