Objective The method of intragastric administration of improved guinea pigs was introduced， which can be used as reference for other guinea pigs gavage. Methods 40 ordinary guinea pigs， male and female half， based on the CCKA receptor-mediated G protein signaling pathway in the gallstones of cholesterol gallstones in the study of the mechanism of the establishment of cholesterol gallstones， after the modeling succeed， the use of the stent into the experimental animal esophageal feeding method（traditional gavage method） and the use of the intragastric needle into the experimental animal mouth into the drug by its own swallowing method（improved aftergrowth method）， the mortality of guinea pigs after intragastric administration of two different gavage methods was monitored. Results Compared with the traditional gavage method， the mortality of the guinea pigs treated with the improved intragastric method was significantly lower（P<0.01）. Conclusion The improved gastric gavage method has high success rate and practicability， so it is worth popularizing.