Objective To investigate the clinical effect of acupuncture at Zusanli and Sanyinjiao acupoints assisted by gynecological surgery in patients with perioperative pain in gynecology. Methods 140 cases of gynecological surgery quasi-scheduled patients according to random number table were divided into control group and treatment group， each group of 70 cases； control group given perioperative anesthesia， the treatment group in the control group on the basis of anesthesia Acupuncture Zusanli， Sanyinjiao two points method to compare the two groups of patients after surgery related indicators and pain. Results The pain VAS scores of the treatment group at 8h， 24h and 48h postoperatively were significantly lower than those of the control group（P<0.05）. The extubation time and eye opening time of the treatment group were significantly lower than those of the control group Significant difference（P<0.05）. Conclusion Perioperative gynecological surgery acupuncture Zusanli， Sanyinjiao assisted anesthesia can significantly reduce postoperative pain and improve the quality of surgery， a reference for the clinical significance.