Objective To confirm the theory of emotion pathopoiesis that basis of TCM， through establishing a rat model of chronic pharyngitis（CP） liver-stagnation and spleen-deficiency CP rat model（GYPXM） to explore the correlation between the occurrence and development of CP and the expression of the key neurotransmitter. Methods 70 wistar rats were used to establishing the CP standard model and liver-stagnation and spleen-deficiency CP rat model， that divided into 7 groups randomly. After modeling， the hypothalamus tissue 5HT mRNA expression in each group was examined， and pathological sections of rats in each group were observed. Results The hypothalamus 5HT mRNA expression in both models was lower than that in the blank group. After 10 days of Yanyanxiao mixture treatment， the 5-HT1B， 5-HT2C receptor two kind of rat model were increased. Conclusion Compared with the standard model ， CP rat model of liver-spleen deficiency established successfully， and is more suitable for clinical and pathological features. The incidence of CP is associated with abnormal expression of the key neurotransmitter which affectded by mood.