Objective To observe the dynamic changes of Chemerin levels in rats with arteriosclerotic cerebral infarction treated by Tongdu Tiaoshen acupuncture method based on the NF-κB pathway and to find the optimal time for acupuncture intervention. Methods Ninety-six adult Wistar male rats were randomly divided into four groups：control group， sham operation group， model group and acupuncture group. According to ischemia-reperfusion 6 h， 24 h， 48 h each is divided into 3 subgroups， 8 in each group. According to the neurological function score of Zea Longa， the standard rat income will be matched to the corresponding group， and the expression activity of NF-KB in peripheral blood mononuclear cells will be determined by flow cytometry. After enzyme-linked immunosorbent assay（ELISA）Determination of Chemerin levels in peripheral blood. Results ①Neurological score of Zea Longa；②Chemerin and NF-κB levels in sham group were not significantly different from those in blank group（P>0.05）；③Chemerin and The level of NF-κB was significantly up-regulated compared with the blank group and the sham operation group（P<0.01）. ④The level of NF-κB began to increase at 6 h after ischemia-reperfusion， and the levels of NF-κB in 24 h and 48 h after ischemia-reperfusion continued to increase. But the level of NF-κB in acupuncture group at 6 h after ischemia-reperfusion was the most significant（P<0.01）. ⑤Chemerin levels in the acupuncture group at 6 h after ischemia-reperfusion were significantly different from those in the model group at the same time point（P<0.01）. The levels of Chemerin in the acupuncture group at 24 h and 48 h after ischemia-reperfusion were not significantly different from those in the model group at the same time point（P>0.05）. Conclusion ①Tongdu Tiaoshen acupuncture can significantly reduce atherosclerosis cerebral infarction Neurological deficit in model rats. ②Tongdu Tiaoshen acupuncture can inhibit NF-κB activity and reduce Chemerin in peripheral blood of rats with atherosclerotic cerebral infarction. Acupuncture at 6 h after ischemia-reperfusion was the best treatment time point.