Objective To observe the influence of rhynchophylline（Rh） on inflammation and related inflammatory factor of TNF-α and IL-1β in dopaminergic neurons and glial cells induced by lipopolysaccharide（LPS）. Methods Dopaminergic neurons and glial cells were extracted， eparated and cultured in 18-day pregnant Wistar rats， and is divided into four groups：control group， LPS model group， the LPS + Rhy group and LPS + NDG group， each group respectively in processing after the completion of 0， 1， 3， 6， 12 h collecting cells and cell culture medium after treated. After immunofluorescence staining， the content of TNF-α and IL-1β in cell culture medium was detected by ELASA. Results The content of the IL-1β and TNF-α went content peak from 3 and 6 hours after treating. Compared with LPS model group， the content of IL-1β and TNF-α were significantly lower in LPS + Rhy group and LPS + NDG（P<0.01）， but there was no statistically significant difference between the group of the LPS + Rh and the LPS + NDG. Conclusion Rh can effectively suppress the content of IL-1β and TNF-α， and then to inhibit the inflammatory and protect dopaminergic neurons.