Objective To investigate the effect of Danggui Buxue Decocton and it’s separate herb on Wnt and Wnt’s receptor in mice with myelosuppression. Methods The mice model of Myelosuppression was established by Cyclophosphamide（CTX）， Automatic Blood Cell Analyzer tested the Peripheral Blood cell number， ELISA tested Wnt3a protein in Bone Marrow， qPCR tested Frizzled 2 mRNA expression in Bone Marrow Hematopoietic Stem Cells. Results Compared to the normal group， the number of WBC， RBC and PLT were decreased significantly in model group（P<0.05 or P<0.01）； The value of Wnt3a protein and Frizzled 2 mRNA were decreased significantly（P<0.01）； Compared to the model group， the number of RBC and PLT were increased significantly in Danggui Buxue Decoction group（P<0.05 or P<0.01）； The value of Wnt3a protein and Frizzled 2 mRNA were increased significantly（P<0.05 or P<0.01）， but the Huangqi group and the Danggui group had no significantly different（P>0.05）. Conclusion First， Danggui Buxue Decoction can increased the value of Wnt3a protein and Frizzled 2 mRNA in Bone Marrow. Second， the regulation of Wnt3a and it’s receptor Frizzled 2 mRNA with Danggui Buxue Decoction and it’s separate herb， showed the correct of “Qi and Blood generate each other” in Traditional Chinese Medicine.